Coding

Part:BBa_K5181008:Design

Designed by: Mayukh Mitra   Group: iGEM24_IISER-TVM   (2024-08-18)


P450_CPR_fusion protein (BC_linker)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 217
    Illegal PstI site found at 895
    Illegal PstI site found at 2686
    Illegal PstI site found at 2965
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 370
    Illegal PstI site found at 217
    Illegal PstI site found at 895
    Illegal PstI site found at 2686
    Illegal PstI site found at 2965
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2349
    Illegal XhoI site found at 1306
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 217
    Illegal PstI site found at 895
    Illegal PstI site found at 2686
    Illegal PstI site found at 2965
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 217
    Illegal PstI site found at 895
    Illegal PstI site found at 2686
    Illegal PstI site found at 2965
    Illegal NgoMIV site found at 346
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amino acid sequence of the part is:

Source

This part is derived from parts (BBa_K5181001) , (BBa_K5181003) and Bifunctional p450 of bacillus subtilus

References